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Temperature-sensitive mutants of bacterial pathogens: isolation and use to determine host clearance and in vivo
1Department of Microbiology, Miami University, Oxford, Ohio 45056.
Methods in Enzymology
|January 1, 1994
Abstract:
Temperature-sensitive mutants of bacterial pathogens are relatively easy to obtain and characterize. We have used ts mutants of a number of bacterial pathogens: E. coli and Listeria monocytogenes to determine quantitatively the bacterial activities in vitro of macrophages and polymorphonuclear leukocytes; E. coli and P. cepacia to study clearance of the bacteria in vivo; and Salmonella enteritidis, Listeria monocytogenes, E. coli, P. aeruginosa, and P. cepacia to determine quantitatively the replication rates in the spleens, lungs, and peritoneal cavities of mice, and the lungs of chickens.