Basic fibroblast growth factor induces c-fos expression in primary cultures of rat striatum

Basic fibroblast growth factor (bFGF) is present in the rat striatum in vivo, where evidence suggests it may have a long-term trophic role in supporting the survival of striatal neurones. To examine the possibility that these effects of bFGF might be mediated by induction of neuronal gene expression, we have investigated the ability of bFGF to stimulate expression of the immediate-early gene c-fos in primary cultures of embryonic rat striatum. The basal levels of c-fos mRNA were low in both neurones and glia in culture. Application of 500 pM bFGF resulted, within 45 min, in a 11-fold increase in the c-fos hybridisation signal in the non-neuronal cells. No significant induction of c-fos mRNA was detected in the striatal neurones at this time. The induction in non-neuronal cells was blocked by the tyrosine kinase inhibitor genistein (100 microM), but not by its inactive structural analogue genistin (100 microM). These results represent a novel mechanism whereby bFGF can exert prolonged effects on striatal function, and indicate that the increases in striatal c-fos gene expression induced by bFGF occur primarily in non-neuronal cells.