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Fyn expression during early neurogenesis in mouse embryos
T Yagi1, Y Shigetani, Y Furuta
1Laboratory of Molecular Oncology, Tsukuba Life Science Center, Ibaraki, Japan.
Oncogene
|September 1, 1994
Summary
Fyn tyrosine kinase is crucial for neural development, showing specific expression patterns in the developing brain and neural crest derivatives. Its non-expression in dividing cells highlights its role in differentiating neural tissues.
Area of Science:
- Developmental Biology
- Neuroscience
- Molecular Biology
Background:
- Fyn, a Src family tyrosine kinase, is implicated in cell-cell interactions during morphogenesis.
- Understanding Fyn's developmental expression is key to elucidating its role in neural development.
Purpose of the Study:
- To investigate the spatiotemporal expression profile of Fyn during embryonic development.
- To characterize Fyn's role in the neural system using a lacZ knock-in mouse model.
Main Methods:
- Utilized a Fyn-lacZ mutant mouse model to track Fyn expression patterns.
- Examined Fyn expression via lacZ gene introduction at various embryonic days (E7.5-E9.5) and later stages.
Main Results:
- Fyn expression was detected early in the headfold (E7.5) and neural groove (E8.5).
- Characteristic regional expression observed in rhombomeres and consistent low-level expression in the notochord.
- High expression in the marginal layer of the neural tube and dorsal root filaments, with no expression in dividing neuroepithelial cells.
Conclusions:
- Fyn expression is dynamically regulated during neural development.
- Fyn's non-expression in dividing cells and expression in neural fibers suggest a role in neural differentiation and fiber development.