Related Experiment Video
Updated: Jul 20, 2026

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
Published on: May 11, 2015
[Diagnosis of experimental diabetes mellitus using isoelectric focusing of hemoglobin]
Abstract:
Glycosylated hemoglobin levels were measured in dogs with experimental diabetes mellitus over two months after hyperglycemia onset. Diabetes was induced by pancreas resection combined with intraarterial alloxan infusion. Modified isoelectrofocusing in PAAG plates was used for glycosylated hemoglobin measurements. Its concentration was found to increase by 55% 4 weeks after diabetes development and to persist growing, making up 183% of its initial level in 2 months. The suggested method of isoelectric focussing in PAAG plates may be used to monitor glycemia in diabetes.
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Type II Diabetes Mellitus III: Clinical Manifestations and Diagnosis

