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Universal phosphorescence immunoassay
Mantrova EYu1, M V Demcheva, A P Savitsky
1Institute of Biochemistry, Russian Academy of Sciences, Moscow.
Analytical Biochemistry
|May 15, 1994
Abstract:
The aim of this study is to develop a universal phosphorescence immunoassay method using monoclonal antibodies to Pd-coproporphyrin (Pd-CP) and conjugates of various proteins with Pd-CP. Pd-CP and monoclonal antibodies obtained allow a convenient method for the determination of various antigens to be developed. The conditions for immunological reactions with Pd-CP were optimized with respect to the components affecting the nonspecific binding Pd-CP and Pd-CP conjugates.