Organic-phase biosensing of secondary alcohols with a Ta. brockii alcohol dehydrogenase electrode

An organic-phase enzyme electrode for monitoring secondary alcohols is described. The new biosensor relies on the biocatalytic activity of Ta. brockii alcohol dehydrogenase in non-aqueous media. The Eastman-AQ 55D polymeric coating, known for its stability in organic solvents, is used to entrap the enzyme and its NADP+ cofactor onto the glassy carbon surface. Various experimental variables, influencing the response of the reagentless sensor, are explored for optimum performance. The solvent-induced changes in the enzymatic activity result in an extended linear range. The work expands the scope of organic-phase biosensors towards additional substrates of analytical significance.