Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specificity and regulation of a synaptic vesicle docking complex

J Pevsner1, S C Hsu, J E Braun

  • 1Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University Medical Center, California 94305.

Neuron
|August 1, 1994
PubMed
Summary

Synaptic vesicle docking involves VAMP, synaptotagmin, syntaxin, and SNAP-25 proteins. A neuron-specific protein, n-sec1, regulates these interactions, suggesting a new model for vesicle docking.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Delivery of multipotent adult progenitor cells via a functionalized plasma polymerized surface accelerates healing of murine diabetic wounds.

Frontiers in bioengineering and biotechnology·2023
Same author

Molecular signatures associated with cognitive deficits in schizophrenia: a study of biopsied olfactory neural epithelium.

Translational psychiatry·2016
Same author

Unique pharmacological actions of atypical neuroleptic quetiapine: possible role in cell cycle/fate control.

Translational psychiatry·2013
Same author

A matrix metalloproteinase inhibitor to treat unresectable cholangiocarcinoma.

HPB : the official journal of the International Hepato Pancreato Biliary Association·2008
Same author

Specific and nonspecific interactions in a molecule with flexible side chain: 2-phenylethanol and its 1:1 complex with argon studied by high-resolution UV spectroscopy.

The Journal of chemical physics·2006
Same author

High-resolution ultraviolet spectroscopy of p-fluorostyrene-water: evidence for a sigma-type hydrogen-bonded dimer.

The Journal of chemical physics·2005

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Synaptic vesicle docking is crucial for neurotransmission.
  • Vesicle docking is mediated by interactions between vesicle proteins (VAMP, synaptotagmin) and plasma membrane proteins (syntaxin, SNAP-25).

Purpose of the Study:

  • To characterize the binding properties of proteins involved in synaptic vesicle docking.
  • To investigate the role of n-sec1 in regulating syntaxin interactions.

Main Methods:

  • Protein binding assays were used to characterize interactions between VAMP, synaptotagmin, syntaxin, and SNAP-25.
  • The binding of n-sec1 to syntaxin and its effect on other protein interactions were analyzed.

Main Results:

Related Experiment Videos

  • SNAP-25 potentiated VAMP 2 binding to syntaxins 1a and 4.
  • n-sec1 bound syntaxin with nanomolar affinity, forming a distinct complex.
  • Recombinant n-sec1 inhibited VAMP or SNAP-25 binding to syntaxin.

    • Conclusions:

    • Syntaxin exists in multiple states, including bound to n-sec1, in 7S particles, and in 20S particles.
    • The syntaxin/n-sec1 complex may precede or regulate the formation of VAMP, SNAP-25, and syntaxin complexes involved in vesicle docking.