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Updated: Aug 3, 2026

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CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
Published on: May 28, 2019
High-throughput rapid yeast DNA extraction. Application to yeast artificial chromosomes as polymerase chain reaction
1Washington University School of Medicine, Center for Genetics in Medicine, St. Louis, Missouri 63110.
Abstract:
An automation-assisted method enables DNA extraction of over a thousand yeast colonies in a day by one person. Yeast DNA is extracted essentially quantitatively in three steps: (a) cells are converted into spheroplasts with yeast lytic enzyme, (b) cells are lysed with proteinase K, and (c) DNA is collected by ethanol precipitation. The DNA stored at 4 degrees C is active in polymerase chain reaction experiments for more than a year.

