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Structural characterization of PNA-DNA duplexes by NMR. Evidence for DNA in a B-like conformation
M Leijon1, A Gräslund, P E Nielsen
1Department of Biophysics, Arrhenius Laboratory, Stockholm University, Sweden.
Biochemistry
|August 23, 1994
Abstract:
The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by 1H NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.