Related Experiment Video
Updated: Oct 3, 2026

In vitro Measurements of Tracheal Constriction Using Mice
Published on: June 25, 2012
Cyclosporine does not affect in vitro bronchial smooth muscle contractions in treated Lewis rats
R Tavakoli1, R C Daly, C G McGregor
1Laboratoire de physiologie respiratoire, UFR Cochin-Port-Royal, Paris, France.
Abstract:
The purpose of this study was to investigate whether cyclosporine treatment could modify airway responses, thus playing a role in bronchial hyperresponsiveness observed in patients after lung transplantation. We have studied the effect of cyclosporine treatment in rats on subsequent bronchial responses to electrical field stimulation, to exogeneous 5-hydroxytryptamine, and acetylcholine in organ baths. The isometric force of contraction of bronchial smooth muscle was measured. Voltage and frequency responses produced by electrical field stimulation were similar in control and in rat bronchi treated with cyclosporine. Concentration-response curves to exogeneous acetylcholine were superimposed for both groups of animals, as were those to 5-hydroxytryptamine. Our results show that cyclosporine treatment does not affect bronchial smooth muscle contraction and rule out possible contribution of cyclosporine to the bronchial hyperresponsiveness described after lung transplantation.
Related Concept Videos
Drugs Used in Lower Respiratory Disorders: Overview
Bronchodilators, the first step of respiration enhancement, come in various forms, each with its own mechanism...
Antiasthma Drugs: β2-Adrenoceptor Agonists
One class of bronchodilators includes β2-adrenoceptor agonists. These agents target the β2-adrenoceptors located on bronchial smooth muscle cells. By stimulating these receptors, β2-agonists induce relaxation in these...
Antiasthma Drugs: Methylxanthines
Theophylline is thought to inhibit phosphodiesterase enzymes, increasing intracellular levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP). This rise in cAMP and cGMP concentrations stimulates cardiac function,...
Antiasthma Drugs: Leukotriene Modifiers
Leukotriene modifiers work through two distinct mechanisms:
Antiasthma Drugs: Muscarinic Receptor Antagonists
Antimuscarinic agents compete with ACh for the same binding site on the muscarinic receptors. By binding to these receptors, they inhibit the downstream effects of ACh and block the parasympathetic...
Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids
ICS work through a multifaceted mechanism of action. They suppress the inflammatory response caused by the proliferation of TH cells. They also reduce the transcription of the IL-2 gene, which is involved in the...

