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Cloning and expression of a cDNA encoding bovine muscarinic acetylcholine m3 receptor
P H Lee1, P K Hodges, F Glickman
1Division of Cell Biology, Burroughs Wellcome Co., Research Triangle Park, NC 27709.
Biochimica Et Biophysica Acta
|August 11, 1994
Abstract:
A cDNA clone encoding bovine muscarinic acetylcholine m3 receptor has been isolated from a bovine brain library. Sequencing of this clone has revealed a single open reading frame encoding a protein of 590 amino acids. Comparison with known muscarinic m3 receptor sequences from other species shows a high degree of conservation (92-98% homology) in the protein sequence. COS cells transfected with this cDNA expressed a single high-affinity (Kd = 43.9 pM) binding site for muscarinic receptor ligand N-[3H]methylscopolamine.