Cloning and structural analysis of calmodulin gene from rice

Full-length cDNA encoding rice (Oryza sativa, cv. Nongken 58S) calmodulin (CaM) mRNA has been cloned using polymerase chain reaction (PCR) with synthesized primers from the sequence of barley CaM gene. Sequence analysis revealed that the rice gene is 450 bp in length that and its predicted polypeptide consists of 148 amino acids. It differs from those that of barley and alfalfa in only one position, respectively. The nucleotide homology between rice and barley is 90%, between rice and alfalfa is 88%, between rice and rat is 73%.