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Microbody (peroxisome) proliferation in mouse kidney induced by methyl clofenapate
Abstract:
Methyl-2-[4-(p-chlorophenyl)-phenoxy]-2-methylpropionate (methyl clofenapate) a hypolipidemic compound, was administered in the diet (0.03%) for 2-5 weeks to male wild type (Cs-a strain) mice. Electron microscopic examination of the kidneys revealed a significant increase in the number of single membrane limited organelles in cells of the P1, P2 and P3 segments of the proximal convoluted tubule. Catalase was localized in these organelles cytochemically by incubating tissue in alkaline 3,3 feet-diaminobenzidine medium; which enabled their identification as peroxisomes. There was no increase in lysosomes in the renal tubules of methy l clofenapate treated animals. It is not certain if the presence of large number of peroxisomes in the proximal tubular epithelium causes impairment of renal function.
Insights
Methyl clofenapate, a hypolipidemic drug, significantly increased peroxisomes in mouse kidney tubules. Further research is needed to determine if this impacts renal function.
Area of Science:
- Biochemistry
- Toxicology
- Cell Biology
Background:
- Methyl clofenapate is a hypolipidemic compound.
- The effects of methyl clofenapate on kidney structure are not fully understood.
Purpose of the Study:
- To investigate the effects of methyl clofenapate on kidney morphology in mice.
- To identify specific organelles affected by methyl clofenapate treatment.
Main Methods:
- Male wild type mice were fed a diet containing 0.03% methyl clofenapate for 2-5 weeks.
- Kidney tissues were examined using electron microscopy.
- Catalase activity was localized cytochemically to identify peroxisomes.
Main Results:
- A significant increase in single membrane-limited organelles, identified as peroxisomes, was observed in proximal convoluted tubule cells (P1, P2, P3 segments).
- No increase in lysosomes was detected in the renal tubules of treated mice.
Conclusions:
- Methyl clofenapate administration leads to a notable proliferation of peroxisomes in mouse renal proximal tubules.
- The functional consequences of this peroxisome increase on renal function remain uncertain.