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Malondialdehyde measurement in urine
M Guichardant1, L Valette-Talbi, C Cavadini
1Nestec, Research Centre Vers-chez-les-Blanc, Lausanne, Switzerland.
Journal of Chromatography. B, Biomedical Applications
|April 22, 1994
Summary
This study introduces an improved method for measuring malondialdehyde (MDA), an indicator of oxidative stress, in urine. The new technique enhances accuracy and detects MDA at picomole levels, revealing significantly elevated levels in burn patients.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Oxidative Stress Research
Background:
- Malondialdehyde (MDA) is a key biomarker for lipid peroxidation.
- Traditional thiobarbituric acid (TBA) assays for MDA lack specificity due to interfering substances.
- Previous HPLC methods for MDA in urine had insufficient sensitivity and resolution.
Purpose of the Study:
- To develop a more sensitive and specific method for quantifying urinary MDA.
- To assess oxidative stress levels in burn patients using the improved MDA assay.
Main Methods:
- Modified the existing HPLC method by replacing TBA with diethylthiobarbituric acid (DETBA).
- Utilized Bakerbond cartridges for isolating MDA-DETBA complexes.
- Quantified MDA using HPLC with fluorescence or ultraviolet detection.
Main Results:
- The improved method allows for specific detection of MDA at picomole levels.
- Urinary MDA levels in burn patients were markedly elevated (mean 18.6 mumol/mmol creatinine) compared to healthy controls (mean 0.23 mumol/mmol creatinine).
- Peak MDA levels in burn patients typically occurred around the third day post-hospitalization and remained high for at least 20 days.
Conclusions:
- The DETBA-based HPLC method provides a sensitive and specific assay for urinary MDA.
- This method is valuable for assessing oxidative stress, particularly in clinical settings like burn care.
- Elevated urinary MDA levels indicate significant oxidative stress in burn patients.