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Updated: Sep 27, 2026

Assaying the Kinase Activity of LRRK2 in vitro
Published on: January 18, 2012
Identification of a latent MAP kinase kinase kinase in PC12 cells as B-raf
1Department of Biochemistry, University of Dundee, Scotland, UK.
Abstract:
A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after stimulation of PC12 cells with nerve growth factor or epidermal growth factor, but this is not accompanied by activation of the Mono Q-purified enzyme whether assayed in the absence or presence of GTP-Ras.
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