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Metalloporphyrin speciation by liquid chromatography and inductively coupled plasma-mass spectrometry
U Kumar1, J G Dorsey, J A Caruso
1Department of Chemistry, University of Cincinnati, Ohio 45221-0172.
Journal of Chromatographic Science
|July 1, 1994
Abstract:
Inductively coupled plasma-mass spectrometry is investigated for the detection of metalloporphyrins (cobalt protoporphyrin, hemin, and zinc protoporphyrin) separated by liquid chromatography. A Hypersil SAS C1 column with mobile phase containing 68% methanol at a pH of 4.5 is used. The detection limits obtained are in the nanogram range for cobalt and zinc protoporphyrins, whereas for hemin, detection limits are in the microgram range. The accuracy of the method is evaluated by determining zinc protoporphyrin from the whole blood of a lead-poisoned patient.