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A sensitive spectrophotometry-based method for the determination of the rate of hydrogen peroxide generation in
M L Di Paolo1, M Scarpa, A Rigo
1Department of Biological Chemistry, University of Padova, Italy.
Journal of Biochemical and Biophysical Methods
|April 1, 1994
Abstract:
A new sensitive spectrophotometric method for the determination of the rate of hydrogen peroxide generation in biological systems has been developed. This method is based on the measurement of the oxidation rate of reduced cytochrome c by H2O2 in the presence of a mediator and permits the detection of H2O2 generation rates as low as 60 nM min-1. The solution of the differential equations of the kinetic process permitted the calculation of the kinetic rate constants and assessment of the conditions required to measure the hydrogen peroxide generation rate.