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Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
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Phaseolus vulgaris lectin labelled and GABA immunogold stained terminals in nucleus rotundus. An EM study

T Tömböl1, G Egedi, A Németh

  • 11st Department of Anatomy, Semmelweis University, Medical School, Budapest, Hungary.

Journal Fur Hirnforschung
|January 1, 1994
PubMed

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.