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Cryopreservation of human hookworms

T J Nolan1, V M Bhopale, Z Megyeri

  • 1Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.

The Journal of Parasitology
|August 1, 1994
PubMed
Summary
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Cryopreservation successfully preserved hookworm larvae, enabling development to infective stages for Ancylostoma duodenale and Necator americanus, with A. duodenale larvae proving infective to dogs.

Area of Science:

  • Parasitology
  • Infectious Diseases
  • Cryobiology

Background:

  • Human hookworm infections caused by Ancylostoma duodenale and Necator americanus are significant global health issues.
  • Effective methods for preserving hookworm larvae are crucial for research and control strategies.

Purpose of the Study:

  • To evaluate the efficacy of cryopreservation for maintaining the viability and infectivity of hookworm larvae.
  • To determine if cryopreserved larvae can develop into infective stages.

Main Methods:

  • First-stage larvae (L1) of A. duodenale and N. americanus were incubated in a cryoprotectant solution (10% DMSO and 10% dextran).
  • Larvae were subsequently frozen in liquid nitrogen and then thawed.
  • Thawed larvae were cultured on agar plates to assess development to the third, infective larval stage (L3).

Related Experiment Videos

  • The infectivity of cryopreserved A. duodenale L3 was tested in dogs.

    • Main Results:

    • Cryopreserved hookworm larvae (L1) successfully developed into infective third-stage larvae (L3) after thawing and culturing.
    • A. duodenale L3 derived from cryopreserved L1 were infective to dogs, demonstrating retained pathogenicity.
    • The infectivity of cryopreserved N. americanus L3 was not assessed in this study.

    Conclusions:

    • Cryopreservation is a viable method for preserving hookworm larvae, allowing for subsequent development to infective stages.
    • This technique supports future research into hookworm biology, drug screening, and vaccine development.
    • The infectivity of cryopreserved A. duodenale larvae highlights the potential for long-term storage and use in experimental infections.