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Determination of basic drugs extracted from biological matrices by means of solid-phase extraction and wide-bore
X H Chen1, J P Franke, J Wijsbeek
1University Center for Pharmacy, Department of Analytical Chemistry and Toxicology, Groningen, The Netherlands.
Abstract:
Determination of basic drugs from biological matrices at concentration levels of 100-200 ng/mL was accomplished by using mixed-mode Bond Elut Certify columns and a gas chromatograph equipped with a nitrogen-phosphorus detector. The extraction procedure developed for general drug screening on a GC-FID system was found suitable for the extraction of basic drugs from plasma and whole blood for GC-NPD analysis. For urine samples, an extra wash step with 20% acetonitrile in water was required to obtain clean extracts. The recoveries of 18 basic drugs ranged from 77.7 to 104.5%, with relative standard deviations less than 7.0%.