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[Study of conditions for cleaving bovine leukemia virus RNA in vitro using ribozymes directed against long terminal
Abstract:
Ribozyme has been constructed by the oligonucleotide synthesis technique that is capable of splitting bovine leukemia virus (BLV) RNA-substrate in the R-region (358th nucleotide). Ribozyme has been shown to efficiently split the RNA containing R-region of the virus. The efficiency of ribozyme function depends on conditions of the reaction reaching its maximum at 25 degrees C and pH 7.3-7.6. The efficiency of splitting is increased by multiple heating-cooling cycles of the substrate-ribozyme mixture.