Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Full-thickness eye wall biopsy. II. In primates

G A Peyman, P Homer, R Kasbeer

    Investigative Ophthalmology
    |July 1, 1975
    PubMed
    Summary

    A new technique allows for intact full-thickness eye wall biopsies in primates. This method yields excellent histologic samples with minimal ocular damage, advancing ophthalmic research.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Modulation of sensitivity to hyperbaric oxygen by CO2 in newborn rats.

    Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·1995
    Same author

    Study of lymphocyte dynamics in the ocular circulation: technique of labeling cells.

    Current eye research·1995
    Same author

    A practical guideline for management of endophthalmitis.

    Ophthalmic surgery·1995
    Same author

    Direction and location of retinotomy for removal of subretinal neovascular membranes.

    Ophthalmic surgery·1995
    Same author

    Posterior segment triple surgery after traumatic eye injuries.

    Ophthalmic surgery·1995
    Same author

    Choroidal hyperpermeability in central serous choroidopathy: a new concept?

    Archives of ophthalmology (Chicago, Ill. : 1960)·1995

    Area of Science:

    • Ophthalmic surgery
    • Primate research
    • Histopathology

    Background:

    • Accurate histologic evaluation of ocular tissues is crucial for understanding eye diseases.
    • Previous biopsy techniques may cause significant ocular damage, limiting their utility.

    Purpose of the Study:

    • To develop and evaluate a novel technique for obtaining intact full-thickness eye wall biopsies in primates.
    • To assess the quality of histologic specimens and the extent of ocular damage associated with the technique.

    Main Methods:

    • Suturing an eye basket for stabilization of the primate eye.
    • Using trephines to create a circumferential scleral incision (4-7 mm).
    • Employing diathermy to deepen the incision to perforation for specimen removal.
    • Covering the resulting defect with a 7 mm scleral homograft.

    Main Results:

    • The technique successfully obtained intact full-thickness eye wall biopsy specimens.
    • Histologic evaluation of the biopsies demonstrated excellent specimen quality.
    • The procedure resulted in minimal damage to the eye wall.

    Conclusions:

    • This novel technique provides a reliable method for acquiring high-quality ocular tissue samples in primates.
    • The procedure minimizes iatrogenic injury, making it suitable for detailed histopathologic analysis in ophthalmic studies.

    Related Experiment Videos