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Adherence of Pasteurella multocida to rabbit respiratory epithelial cells in vitro
L F Bonilla-Ruz1, G A García-Delgado
1Sección de Microbiología y Parasitología, Centro Interdisciplinario de Ciencias de la Salud, Instituto Politécnico Nacional, Edo. de México, Mexico.
Abstract:
Adult clinically healthy New Zealand rabbits were sampled bacteriologically to detect carriers and non-carriers of Pasteurella multocida. Both groups of rabbits were killed separately to obtain samples of nasal, buccal, pharyngeal and tracheal epithelial cells. The cells were tested for adherence in vitro to 18 isolates of P. multocida from healthy and sick rabbits, from ovine, bovine, cat and swine. The number of bacteria adhered per cell up to 25 cells per preparation were registered. Analysis of variance was used to interpret the significance of results. Adherence of P. multocida was significantly higher to carrier rabbit cells than to non-carrier rabbit cells. Bacterial isolates from rabbits were more adherent to rabbit cells than to isolates from other species. The frequency was higher to buccal and pharyngeal cells than to nasal and tracheal cells. Isolates from healthy animals adhered better to rabbit cells than isolates from sick animals, except the isolates from sick animals which adhered better to nasal cells of non carriers than did isolates from healthy rabbits.
Insights
Pasteurella multocida bacteria adhere more readily to epithelial cells from carrier rabbits. This study investigated bacterial adherence to rabbit cells, finding variations based on host carrier status and bacterial origin.
Area of Science:
- Veterinary Microbiology
- Animal Bacteriology
- Infectious Disease Research
Background:
- Pasteurella multocida is a significant pathogen in various animal species.
- Understanding bacterial adherence is crucial for controlling infections.
- Rabbit epithelial cells serve as a model for studying host-pathogen interactions.
Purpose of the Study:
- To investigate the adherence of Pasteurella multocida to epithelial cells from carrier and non-carrier rabbits.
- To compare the adherence of P. multocida isolates from different animal species and health statuses to rabbit cells.
- To identify specific epithelial cell types and host factors influencing P. multocida adherence.
Main Methods:
- Bacteriological sampling of healthy New Zealand rabbits to identify Pasteurella multocida carriers.
- In vitro adherence assays using epithelial cells (nasal, buccal, pharyngeal, tracheal) from carrier and non-carrier rabbits.
- Testing adherence of 18 P. multocida isolates from various sources (rabbits, sheep, cattle, cats, swine) and health statuses.
- Quantitative analysis of bacterial adherence per cell using analysis of variance.
Main Results:
- Pasteurella multocida exhibited significantly higher adherence to epithelial cells from carrier rabbits compared to non-carrier rabbits.
- Rabbit-origin P. multocida isolates showed greater adherence to rabbit epithelial cells than isolates from other species.
- Buccal and pharyngeal cells demonstrated higher adherence frequencies than nasal and tracheal cells.
- Isolates from healthy animals adhered better to rabbit cells, with a notable exception for sick animal isolates adhering better to non-carrier nasal cells.
Conclusions:
- Host carrier status significantly influences Pasteurella multocida adherence to rabbit epithelial cells.
- Rabbit-specific bacterial isolates possess enhanced adherence capabilities to homologous host cells.
- Epithelial cell location (buccal, pharyngeal) plays a role in bacterial adhesion patterns.
- Further research into host-specific adherence mechanisms could inform strategies for controlling Pasteurella multocida infections.