Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Melanocyte halo explained

M Tata1, G S Sidhu

  • 1Department of Pathology, New York University School of Medicine, New York.

Ultrastructural Pathology
|May 1, 1994
PubMed
Summary
This summary is machine-generated.

Melanocyte halos in skin biopsies are an artifact caused by paraffin embedding. High temperatures during this process likely create the observed retraction artifact around melanocytes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The impact of anaemia and iron deficiency in chronic obstructive pulmonary disease: A clinical overview.

Revista portuguesa de pneumologia·2017
Same author

Short communication: Thermostability of α-amylase produced by Bacillus sp. E2-a thermophilic mutant.

World journal of microbiology & biotechnology·2014
Same author

Genetic analysis of brown planthopper resistance in twenty varieties of rice, Oryza saliva L.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Genetic analysis of bacterial blight resistance in seventy-four cultivars of rice, Oryza sativa L.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Effect of felodipine on the serum lipid profile of patients with hypertension.

Indian journal of clinical biochemistry : IJCB·2012
Same author

The removal of acetabular bone in hip resurfacing and cementless total hip replacement: a comparison using the ratio of the size of the acetabular component to the diameter of the native femoral head.

The Journal of bone and joint surgery. British volume·2012
Same journal

Autism spectrum disorder (ASD): zinc suppresses cerebellar ultrastructural alterations and modulates the oxidative stress/gliosis/neuronal loss axis-mediated ASD.

Ultrastructural pathology·2026
Same journal

Alteration of adult neural stem cells by gold nanoparticles in a demyelination model induced in the hippocampus of adult male mice: a structural and ultrastructural study.

Ultrastructural pathology·2026
Same journal

The potential neuroprotective effect of apigenin on cerebellar cortex alterations in valproic acid-induced autism model of male albino rat pups: a histological and immunohistochemical study.

Ultrastructural pathology·2026
Same journal

Deposits of C3 glomerulopathies have ultrastructural hints to correlate with immunofluorescent findings.

Ultrastructural pathology·2026
Same journal

Effect of concurrent administration of granulocyte colony stimulating factor and pirfenidone on experimentally induced lung fibrosis in adult male albino rats (a histological, immunohistochemical & ultrastructural study).

Ultrastructural pathology·2026
Same journal

From lamellar and storage bodies to mitophagy: a comprehensive view of mitochondrial ultrastructure in differentiated SH-SY5Y cells.

Ultrastructural pathology·2026
See all related articles

Area of Science:

  • Dermatopathology
  • Electron Microscopy
  • Histology

Background:

  • Melanocytes are crucial epidermal cells involved in pigmentation.
  • Electron microscopy (EM) is a high-resolution imaging technique used in pathology.
  • Standard tissue processing for EM can introduce artifacts.

Purpose of the Study:

  • To investigate the origin of retraction artifacts observed around melanocytes in electron microscopy.
  • To determine if tissue processing steps, specifically paraffin embedding, contribute to these artifacts.

Main Methods:

  • Skin biopsy specimens were prepared for electron microscopy using three different protocols.
  • Protocols included direct processing after fixation, retrieval from paraffin blocks, and processing with xylene clearing before paraffin infiltration.

Related Experiment Videos

  • All specimens underwent electron microscopic examination.

    • Main Results:

    • A distinct halo, a retraction artifact, was consistently observed surrounding melanocytes.
    • This artifact was associated with the paraffin infiltration step during tissue processing.
    • The artifact appeared to be exacerbated by the high temperatures of the paraffin bath.

    Conclusions:

    • The halos observed around melanocytes in electron microscopy are a processing artifact, not a true biological feature.
    • Paraffin embedding, particularly the high temperatures involved, is the likely cause of this retraction artifact.
    • Understanding this artifact is crucial for accurate histopathological interpretation of melanocyte morphology.