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Differentiation analysis of bovine T-lymphosarcoma
N Ishiguro1, T Matsui, M Shinagawa
1Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Veterinary Immunology and Immunopathology
|May 1, 1994
Summary
Sporadic bovine leukosis (SBL) tumor cells were analyzed for T-cell receptor (TCR) gene expression. Results indicate these cells are immature double-negative T-cells with non-functional TCR gamma delta transcripts.
Area of Science:
- Veterinary Pathology
- Immunology
- Oncology
Background:
- Sporadic bovine leukosis (SBL) is a lymphosarcoma affecting cattle.
- Understanding the T-cell lineage and differentiation of SBL tumor cells is crucial for disease characterization.
Purpose of the Study:
- To investigate the immunophenotypic profile, T-cell receptor (TCR) gene expression, and clonality of SBL tumor cells.
- To determine the differentiation stage and lineage of SBL-derived lymphoid tumor lines.
Main Methods:
- Transplantation of SBL tumor cells (calf, skin, thymic types) into nude mice to establish lymphoid tumor lines.
- Flow cytometry analysis (FACS) using monoclonal antibodies (mAbs) for cell surface markers.
- Examination of T-cell receptor (TCR) gene rearrangement and transcript expression using molecular techniques.
Main Results:
- Tumor cells exhibited alpha-naphthyl acetate esterase positivity and lacked surface immunoglobulin (sIg).
- FACS analysis revealed a BoCD5+, BoCD6+, BoCD4-, BoCD8- immunophenotype.
- TCR-beta and -delta gene rearrangement confirmed T-cell clonality, while TCR-alpha and -gamma genes were not rearranged.
- Non-functional, mature or truncated TCR-beta, -gamma, and -delta transcripts were detected.
Conclusions:
- SBL tumor cells represent immature double-negative T-cells.
- These cells express non-functional TCR gamma delta transcripts, suggesting a specific differentiation pathway.