[Evaluation of platelet quality obtained using conventional methods or from buffy-coat and stored at room...

A comparison has been made between platelet concentrates (Pcs) obtained from buffy coat (Bc) and from platelet rich plasma. The method of preparation proves to have an platelet properties in vitro. Both types of Pcs were investigated immediately after isolation as well as during the 5 day period of storage. It has been confirmed that due to the new technique the number of white cells in Pcs could be reduced 25 fold (to 2.79 +/- 1.68 x 10(6)/unit). It has also been showed that traditional way of obtaining Pcs provokes a greater platelet activation than the new technique. The storage conditions of Pcs obtained from Bc must however be practically defined because when CLX containers are used, afer 5 days an excessive pH increase (to 7.59), aggregation decline and HSR weakening are observed.