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[Use of modified acid elution technique in laboratory diagnosis of autoimmune hemolytic anemia]
J Fabijańska-Mitek1, G Kuśnierz-Alejska
1Zakładu Serologii Instytutu Hematologii i Transfuzjologii w Warszawie.
Three modifications of acid elution method were used for the diagnosis of autoimmune haemolytic anaemia. These procedures were adapted to obtain more potent eluates and to prepare intact antibody-free red blood cells for phenotyping and for autologous absorption of warm-reactive autoantibodies. From the results presented in this paper we conclude that the modifications of the acid elution method are better than the up to now used procedure with a mixture of proteolytic enzyme and a thiol reagent. Moreover, these procedures are quick and easy to perform as well as more efficient. The obtained colourless eluates can be investigated in a gel-lest.
Three modifications of acid elution method were used for the diagnosis of autoimmune haemolytic anaemia. These procedures were adapted to obtain more potent eluates and to prepare intact antibody-free red blood cells for phenotyping and for autologous absorption of warm-reactive autoantibodies. From the results presented in this paper we conclude that the modifications of the acid elution method are better than the up to now used procedure with a mixture of proteolytic enzyme and a thiol reagent. Moreover, these procedures are quick and easy to perform as well as more efficient. The obtained colourless eluates can be investigated in a gel-lest.