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Salivary theophylline concentrations in neonates
1Department of Clinical Biochemistry, King's College Hospital, Denmark Hill, London, UK.
Annals of Clinical Biochemistry
|May 1, 1994
Summary
Saliva accurately reflects theophylline levels in neonates, matching serum concentrations. This finding supports using salivary theophylline measurements as a reliable alternative to serum tests in infants.
Area of Science:
- Neonatal pharmacology
- Analytical chemistry
- Biomarker analysis
Background:
- Theophylline is a crucial medication for neonatal respiratory conditions.
- Accurate therapeutic drug monitoring is essential for optimizing efficacy and minimizing toxicity.
- Non-invasive methods for drug level assessment are highly desirable in neonates.
Purpose of the Study:
- To evaluate the correlation between salivary and serum theophylline concentrations in neonates.
- To assess the reliability of homogeneous enzyme immunoassay for measuring salivary theophylline.
- To compare salivary theophylline levels with free serum theophylline concentrations.
Main Methods:
- Measurement of salivary theophylline concentrations using homogeneous enzyme immunoassay.
- Comparison of salivary theophylline with total and free serum theophylline concentrations.
- Validation of immunoassay results against high-performance liquid chromatography (HPLC).
Main Results:
- Salivary theophylline concentrations closely mirrored serum theophylline levels.
- Free serum theophylline concentrations showed near-identical values to salivary concentrations.
- Homogeneous enzyme immunoassay results demonstrated strong agreement with HPLC methods.
Conclusions:
- Saliva is a viable and accurate matrix for monitoring theophylline levels in neonates.
- Homogeneous enzyme immunoassay provides a reliable method for salivary theophylline determination.
- Salivary theophylline measurement offers a non-invasive alternative for therapeutic drug monitoring in infants.