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Molecular characterization of the gene encoding high-level mupirocin resistance in Staphylococcus aureus J2870
J E Hodgson1, S P Curnock, K G Dyke
1Department of Biotechnology, SmithKline Beecham Pharmaceuticals, Brockham Park, Betchworth Surrey, United Kingdom.
Abstract:
The nucleotide sequence of the ileS gene conferring high-level resistance to mupirocin in Staphylococcus aureus J2870 has been determined. The gene sequence is substantially different from that of the native ileS gene of S. aureus, indicating that high-level resistance to mupirocin results from the acquisition of a novel ileS gene.
Insights
High-level mupirocin resistance in Staphylococcus aureus J2870 is due to a novel ileS gene. This acquired gene differs significantly from the native S. aureus ileS gene, explaining the resistance mechanism.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Staphylococcus aureus is a common pathogen.
- Mupirocin is an important antibiotic for treating S. aureus infections.
- Antibiotic resistance is a growing public health concern.
Purpose of the Study:
- To determine the nucleotide sequence of the ileS gene responsible for high-level mupirocin resistance in Staphylococcus aureus J2870.
- To compare the novel ileS gene sequence with the native S. aureus ileS gene.
Main Methods:
- Nucleotide sequencing of the ileS gene from S. aureus J2870.
- Bioinformatic analysis and comparison of gene sequences.
Main Results:
- The nucleotide sequence of the ileS gene conferring high-level mupirocin resistance was determined.
- The identified ileS gene sequence is substantially different from the native S. aureus ileS gene sequence.
Conclusions:
- High-level mupirocin resistance in Staphylococcus aureus J2870 is acquired through a novel ileS gene.
- The acquisition of this novel ileS gene is the basis for high-level mupirocin resistance in this strain.