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Related Experiment Videos

PEBP2--a modulator of polyoma DNA replication

S E Rutberg1, L R Dolan, Z Ronai

  • 1Molecular Carcinogenesis Program, American Health Foundation, Valhalla, NY 10595.

DNA and Cell Biology
|August 1, 1994
PubMed
Summary

UV irradiation represses polyoma DNA replication in mouse cells, unlike in rat cells. This effect is mediated by PEBP2 binding proteins, suggesting their role in DNA damage response.

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Area of Science:

  • Molecular Biology
  • Virology
  • Cellular Biology

Background:

  • Integrated polyoma DNA replication can be induced by DNA-damaging agents in rat fibroblasts (H3 cells).
  • The cellular response to DNA damage involves complex regulatory mechanisms affecting viral replication.

Purpose of the Study:

  • To investigate the effect of UV irradiation on polyoma DNA replication in mouse fibroblasts (WOP cells).
  • To identify the role of PEBP2 binding proteins in mediating UV-induced changes in polyoma replication.

Main Methods:

  • Transient transfection of WOP cells with polyoma DNA.
  • UV irradiation of transfected cells.
  • Cotransfection with wild-type and mutated PEBP2 target sequences.
  • DNA-binding assays to compare nuclear protein complexes.

Main Results:

  • UV irradiation repressed polyoma replication in WOP cells.
  • Restoration of polyoma replication was observed with wild-type, but not mutated, PEBP2 target sequences.
  • Differential formation of PEBP2 binding complexes in UV-irradiated WOP and H3 cells was detected.

Conclusions:

  • PEBP2 binding proteins play a role in mediating the repression of polyoma replication following UV irradiation in WOP cells.
  • The activity of PEBP2 binding proteins is differentially regulated by UV irradiation in different cell types.
  • PEBP2 proteins may be involved in the cellular response to DNA damage.

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