Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microdissection by ultrasonication: application to early chick embryos

F N Low1, S G McClugage

  • 1Department of Anatomy, Louisiana State University Medical Center, New Orleans 70112.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|May 1, 1994
PubMed
Summary

Ultrasonication effectively microdissects early chick embryos for scanning electron microscopy. This technique reveals structural details of embryonic development, preserving germ layer relationships.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Some characteristics of peripheral visual performance.

The American journal of physiology·2010
Same author

The development of peripheral visual acuity during the process of dark adaptation.

The American journal of physiology·2010
Same author

An anomalous middle meningeal artery.

The Anatomical record·2010
Same author

The peripheral visual acuity of 100 subjects under scotopic conditions.

The American journal of physiology·2010
Same author

The peripheral motion acuity of 50 subjects.

The American journal of physiology·2010
Same author

Analysis of graft healing in a new elastomer-coated vascular prosthesis.

Cardiovascular surgery (London, England)·1993

Area of Science:

  • Developmental Biology
  • Microscopy Techniques
  • Scanning Electron Microscopy

Background:

  • Studying early embryonic development requires advanced imaging techniques.
  • Conventional methods may obscure fine structural details crucial for understanding morphogenesis.

Purpose of the Study:

  • To evaluate ultrasonication as a microdissection technique for scanning electron microscopy of early chick embryos.
  • To characterize the fragmentation patterns and structural integrity of embryonic tissues under sonication.

Main Methods:

  • Chick embryos (first three days of incubation) were subjected to microdissection using ultrasonication (80 kHz tank cleaner in acetone).
  • Embryos were sonicated for varying durations, and fragmentation patterns were observed.
  • A comparative analysis was performed on embryos treated with collagenase prior to sonication and fixation.

Related Experiment Videos

Main Results:

  • Ultrasonication rapidly fragmented 12-hour embryos (≤1 second).
  • Fragmentation required longer sonication times (10-20 seconds to 1 minute+) for later developmental stages (18 hours through day 3).
  • Germ layers (ectoderm, endoderm, mesoderm) maintained structural integrity and relative positions throughout sonication; collagenase pre-treatment destroyed lamellipodia and filopodia.

Conclusions:

  • Ultrasonication is a viable method for microdissecting early chick embryos for SEM.
  • The technique preserves fundamental embryonic structures, offering unique insights into developmental processes.
  • Factors like extracellular matrix composition and cell junctions influence fragmentation patterns.