High-performance liquid chromatographic separations of nystatin and their influence on the antifungal activity

Several efficient analytical HPLC methods for the separation and comparison of the nystatin complex have been developed. These separations have been extended to the preparative scale in order to gain sufficient quantities of the purified nystatin components for testing antifungal activity. For the first time we describe a new HPLC method which does not affect the antifungal activity of nystatin.