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Determination of sterol content in different food samples by capillary gas chromatography
M Rodríguez-Palmero1, S de la Presa-Owens, A I Castellote-Bargallo
1Department de Ciències Fisiològiques i de la Nutrició, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Journal of Chromatography. A
|June 24, 1994
Abstract:
An accurate method for the determination of sterols by capillary gas chromatography was developed and applied to the analysis of food. The procedure includes the following steps: dichloromethane-methanol (2:1, v/v) lipid extraction, saponification at 80 degrees C and separation of the unsaponifiable matter with cyclohexane, derivatization to form trimethylsilyl ethers and gas chromatography using 5 alpha-cholestane as the internal standard. The method shows good accuracy, precision and sensitivity and is suitable for the determination of sterols in food.