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Rapid and accurate random urinary porphyrin quantitation
1Department of Pediatrics, Medical College of Virginia, Richmond 23298.
Summary
Random urine profiling accurately assesses porphyrin excretion, matching 24-hour collections. This method simplifies analysis for organic acids and porphyrins, offering a rapid initial evaluation.
Area of Science:
- Clinical Chemistry
- Analytical Biochemistry
- Metabolomics
Background:
- 24-hour urine collections are standard for porphyrin analysis but are difficult to obtain accurately.
- Porphyrins are susceptible to degradation, posing challenges for accurate quantification.
Purpose of the Study:
- To evaluate the utility of random urine void analysis for porphyrin profiling.
- To compare results from random voids with traditional 24-hour collections.
- To investigate the impact of alkalinizing agents and diurnal variations on porphyrin analysis.
Main Methods:
- Analysis of porphyrin patterns in randomly voided urine samples from normal adults.
- Comparison with results from 24-hour urine collections.
- Evaluation of different alkalinizing agents (e.g., NaOH) and diurnal excretion patterns.
Main Results:
- Sodium hydroxide (NaOH) was found to stabilize porphyrins, optimizing uroporphyrin recovery.
- Porphyrin profiles from random urine voids closely correlated with those from 24-hour collections.
- No significant influence of diurnal variations on random urine void results was observed.
Conclusions:
- Random urine profiling provides a rapid and accurate method for initial porphyrin evaluation.
- This approach simplifies sample collection compared to 24-hour methods.
- Normal reference values for random urine porphyrin analysis in males and females were established.