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The recetor sites for complement (C3) on human diploid fibroblasts
Virchows Archiv. B, Cell Pathology
|January 1, 1975
Summary
Human diploid fibroblasts (WI-38 cells) form rosettes with complement-coated sheep red blood cells (EAC), indicating immune adherence. WI-38 cells possess complement receptors, likely for C3, on their surface.
Area of Science:
- Immunology
- Cell Biology
- Microscopy
Background:
- Immune adherence is a phenomenon where cells bind to antibody- or complement-coated particles.
- Human diploid fibroblasts (WI-38 cells) are a model for studying cell-surface interactions.
Purpose of the Study:
- To investigate the interaction between sheep red blood cells treated with complement (EAC) and human diploid fibroblasts (WI-38 cells).
- To determine if WI-38 cells possess receptors for complement components.
Main Methods:
- Scanning electron microscopy was used to visualize the interaction.
- Sheep red blood cells sensitized with antiserum and complement (EAC) were incubated with WI-38 cells.
- Control experiments used unsensitized or antibody-sensitized red blood cells (E or EA).
Main Results:
- EAC formed rosettes with WI-38 cells, attaching to microvilli, not directly to the cell surface.
- Unsensitized or antibody-sensitized red blood cells (E or EA) did not attach to WI-38 cells.
- Pretreatment of WI-38 cells with mouse serum did not inhibit EAC binding, and no phagocytosis was observed.
Conclusions:
- The interaction between WI-38 cells and EAC is consistent with immune adherence.
- WI-38 cells likely express complement receptors, particularly for C3, on their cell membrane.