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Two new proteins preferentially associated with membrane immunoglobulin D
K M Kim1, T Adachi, P J Nielsen
1Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
The EMBO Journal
|August 15, 1994
Summary
Researchers identified novel proteins linked to membrane IgD and IgM on B cells. These findings may explain functional differences between these crucial antigen receptors.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- B cells utilize IgM and IgD antigen receptors with distinct functions.
- The intracellular components linking these receptors to the cytoplasm remain largely unknown.
Purpose of the Study:
- To identify novel intracellular membrane-associated proteins interacting with B cell antigen receptors.
- To investigate potential molecular links between membrane IgD and IgM and cellular functions.
Main Methods:
- A novel biotinylation protocol was developed to isolate intracellular membrane-associated proteins.
- Proteins associated with membrane IgD (mIgD) and membrane IgM (mIgM) were purified and sequenced.
Main Results:
- Two novel, related proteins (29 and 31 kDa) were found associated with mIgD and, to a lesser extent, mIgM.
- mIgM was specifically associated with three other proteins (32, 37, and 41 kDa).
Conclusions:
- The identified novel proteins may serve as a link between antigen receptors and the cytoskeleton.
- These proteins could elucidate the functional divergence between mIgM and mIgD on B cells.