Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evaluation of HBsAg detection methods from AABB-CAP survey data

H F Polesky, H F Taswell

    American Journal of Clinical Pathology
    |June 1, 1975
    PubMed
    Summary

    Radioimmunoassay (RIA) identified more Hepatitis B surface antigen (HBsAg) positive samples than counterelectrophoresis (CEP). While RIA had initial specificity issues, both methods improved over two years, with CEP performance enhancing significantly.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inbreeding, heterozygosity, and lymphoma risk among the baboons (Papio hamadryas) of Sukhumi, USSR.

    American journal of primatology·2020
    Same author

    Presensitization patterns in renal allograft recipients-A correlation with graft outcome.

    Irish journal of medical science·2016
    Same author

    The use and abuse of the full-sibling and half-sibling indices.

    Transfusion·2000
    Same author

    Impact of molecular (DNA) testing on determination of parentage.

    Archives of pathology & laboratory medicine·1999
    Same author

    Improving the safety of transfusion therapy.

    Clinics in laboratory medicine·1996
    Same author

    How much fresh, low-titer, O-negative blood is available?

    Transfusion·1996

    Area of Science:

    • Clinical chemistry
    • Immunology
    • Hepatitis B diagnostics

    Background:

    • Hepatitis B surface antigen (HBsAg) detection is crucial for diagnosing Hepatitis B virus infection.
    • Various serological methods exist for HBsAg detection, each with unique performance characteristics.
    • Inter-laboratory comparisons are essential for evaluating diagnostic assay performance and consistency.

    Purpose of the Study:

    • To review and compare the performance of HBsAg detection methods used in a survey.
    • To analyze trends in method popularity and participant performance over a two-year period.
    • To evaluate the effectiveness of counterelectrophoresis (CEP) and radioimmunoassay (RIA) for HBsAg detection.

    Main Methods:

    • Analysis of results from nine HBsAg survey panels.
    • Comparison of participant data for counterelectrophoresis (CEP) and radioimmunoassay (RIA).
    • Evaluation of method sensitivity, specificity, and performance changes over time.

    Main Results:

    • Radioimmunoassay (RIA) consistently identified a higher number of HBsAg positive samples compared to CEP.
    • Initial specificity challenges with RIA were observed but improved over the two-year survey period.
    • Participant performance using CEP demonstrated improvement throughout the study duration.
    • The survey documented a shift in the adoption and popularity of different HBsAg detection methods.

    Conclusions:

    • RIA offers superior sensitivity for HBsAg detection compared to CEP.
    • Continuous monitoring and quality assurance can mitigate specificity issues in diagnostic assays.
    • Ongoing training and participation in proficiency testing can enhance laboratory performance for methods like CEP.
    • The findings highlight the evolving landscape of Hepatitis B diagnostic methodologies.

    Related Experiment Videos