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Updated: Aug 3, 2026

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
Published on: January 18, 2014
Modulation of the spc operon affects growth and protein secretion in Bacillus subtilis
R Breitling1, B Schlott, D Behnke
1Friedrich-Schiller-Universität Jena, Biologisch-Pharmazeutische Fakultät, Institut für Molekularbiologie, Germany.
Abstract:
A proximal segment of B. subtilis secY gene was placed under the control of the inducible spac promoter/Lac repressor system. This fusion was integrated into the chromosomal spc operon of B. subtilis via Campbell-like reciprocal recombination. The growth of the resulting strain was strongly IPTG dependent. With staphylokinase and alpha-amylase as reporter proteins it was found, that the protein secretion capacity of this strain was correlated to the conditions of repression or induction of the chromosomal spac promoter.
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