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Carbon disulfide in blood: a method for storing and analysing samples
L Perbellini1, G Maranelli, F Lombardini
1Institute of Occupational Medicine, University of Verona, Policlinico Borgo Roma, Italy.
La Medicina Del Lavoro
|March 1, 1994
Summary
Carbon disulfide (CS2) levels in human blood decrease significantly during storage at 4°C. Fresh blood analysis is crucial for accurate CS2 measurements, with bound CS2 being much higher than free CS2.
Area of Science:
- Toxicology
- Analytical Chemistry
- Biochemistry
Background:
- Carbon disulfide (CS2) is a solvent with potential health implications.
- CS2 exists in blood in both free and bound (acid-labile) forms.
- Disulfiram treatment can increase CS2 levels due to biotransformation.
Purpose of the Study:
- To quantify free and acid-labile carbon disulfide in human blood.
- To assess the impact of sample storage conditions on CS2 concentrations.
- To compare CS2 levels in occupationally unexposed individuals and those treated with disulfiram.
Main Methods:
- Gas chromatography-mass spectrometry (GC-MS) was employed for CS2 analysis.
- Blood samples from 62 unexposed subjects and 27 disulfiram-treated subjects were analyzed.
- Comparison of CS2 concentrations in fresh versus stored blood samples (4°C and -80°C).
Main Results:
- Stored blood samples (4°C for 15-40 days) showed significant CS2 degradation (free CS2 decreased to 26%, acid-labile to 27%).
- In fresh blood, median free CS2 was 139 ng/l and median acid-labile CS2 was 2743 ng/l.
- Free and acid-labile CS2 concentrations were highly correlated (r = 0.9358).
- Blood stored at -80°C maintained stable CS2 concentrations for nearly three weeks.
Conclusions:
- Sample storage at 4°C leads to substantial degradation of both free and acid-labile carbon disulfide in human blood.
- Accurate quantification of carbon disulfide requires analysis of fresh blood samples.
- Cryopreservation at -80°C appears suitable for preserving carbon disulfide levels in blood samples.