Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescence induction in intact spinach chloroplasts

R C Jennings, G Forti

    Biochimica Et Biophysica Acta
    |July 8, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Real-life efficacy and safety of Ustekinumab as second- or third-line therapy in Crohn's disease: results from a large Italian cohort study.

    European review for medical and pharmacological sciences·2021
    Same author

    EAA clinical practice guidelines-gynecomastia evaluation and management.

    Andrology·2019
    Same author

    Clinical impact of the new SIAPEC-IAP classification on the indeterminate category of thyroid nodules.

    Journal of endocrinological investigation·2018
    Same author

    Short-term FSH treatment and sperm maturation: a prospective study in idiopathic infertile men.

    Andrology·2017
    Same author

    Gender identity, gender assignment and reassignment in individuals with disorders of sex development: a major of dilemma.

    Journal of endocrinological investigation·2016
    Same author

    Lower bone turnover and relative bone deficits in men with metabolic syndrome: a matter of insulin sensitivity? The European Male Ageing Study.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2016
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    This study identifies a specific dip in the fluorescence-induction curve of spinach chloroplasts, likely caused by photochemical quenching during photosynthesis. This phenomenon provides insights into electron transport components and protein involvement.

    Area of Science:

    • Photosynthesis research
    • Plant biochemistry
    • Chloroplast function

    Background:

    • Photosystem II (PSII) plays a crucial role in light-dependent reactions of photosynthesis.
    • Fluorescence induction curves are valuable tools for studying the dynamics of PSII.
    • Understanding electron transport in chloroplasts is key to plant physiology.

    Purpose of the Study:

    • To investigate the cause of a pronounced dip in the fluorescence-induction curve of spinach chloroplasts.
    • To characterize the conditions and factors influencing this fluorescence dip.
    • To elucidate the role of specific electron transport components in this phenomenon.

    Main Methods:

    • Measurement of chlorophyll fluorescence induction curves in intact spinach chloroplasts.

    Related Experiment Videos

  • Treatment with dithionite to reduce the plastoquinone pool.
  • Inhibition studies using 3(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU).
  • Modulation of conditions using far-red light, methylviologen, anaerobiosis, and uncouplers.

    • Main Results:

    • A pronounced dip was observed in the fluorescence-induction curve under specific conditions (rapid PSII quencher reduction).
    • This dip, potentially the 'I-D dip', was inhibited by DCMU and occurred with a reduced plastoquinone pool.
    • The dip's behavior under various treatments suggests photochemical quenching by an electron transport component between the PSII quencher and plastoquinone.

    Conclusions:

    • The observed fluorescence dip represents a photochemical quenching mechanism.
    • This quenching involves an electron transport component located between the Photosystem II quencher and plastoquinone.
    • Glutaraldehyde inhibition suggests that protein structural changes may be implicated in this quenching process.