[Localization and identification of the LH-RF center in the brain of the toad, Bufo vulgaris Laur]

Using indirect immunofluorescence procedure with an anti-synthetic LHRF serum, pericarya of neurons producing LHRF or an LHRF-like substance were revealed in hypophysectomized Bufo vulgaris. They are localized principally in the forebrain near the posterior end of the first and second ventricles. The fluorescent axons coming from these pericarya are visible on their pathway to a region near tha pars tuberalis and to the median eminence.