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[Delta 5-3 beta hydroxysteroid dehydrogenase activity in the human full term placenta after culture: the effect of
The study made by histochemistry shows that the delta5-3 beta-hydroxysteroid-dehydrogenase (delta5-3 beta-HSDH) activity which is positive in the fresh placental tissue disappears after 24 h of culture but is maintained much longer if culture medium is supplemented with human chorionic gonadotropin (15 UI of HCG). The biochemical study confirms the delta5-3 beta-HSDH activity decrease during the first 24 h of culture. The enzymatic activity is restored during the following six days and this phenomenon is stimulated by the addition of HCG in the culture medium.
The study made by histochemistry shows that the delta5-3 beta-hydroxysteroid-dehydrogenase (delta5-3 beta-HSDH) activity which is positive in the fresh placental tissue disappears after 24 h of culture but is maintained much longer if culture medium is supplemented with human chorionic gonadotropin (15 UI of HCG). The biochemical study confirms the delta5-3 beta-HSDH activity decrease during the first 24 h of culture. The enzymatic activity is restored during the following six days and this phenomenon is stimulated by the addition of HCG in the culture medium.