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Analysis of filamin-actin binding and cross-linking/bundling by kinetic method
W H Goldmann1, R Senger, G Isenberg
1Technical University of Munich, Garching, FRG.
Biochemical and Biophysical Research Communications
|August 30, 1994
Abstract:
The reaction of smooth muscle filamin and skeletal muscle actin was kinetically examined by double exponential analysis. The overall rate of binding, k+1, is concentration and temperature dependent whilst the overall rate of cross-linking/bundling, k+2, is concentration independent. The activation energy, Ea = 99.5 kJ/mol, was calculated from the Arrhenius Plot.