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Nuclear orphan receptor-binding retinoic acid response elements in keratinocytes
1Center for Nutrition and Toxicology, NOVUM, Karolinska Institute, Sweden.
Biochemical and Biophysical Research Communications
|August 30, 1994
Summary
This study shows that ARP subfamily proteins in keratinocytes bind to specific retinoic acid response elements (RAREs). This binding suggests these proteins may influence retinoic acid signaling in skin.
Area of Science:
- Molecular Biology
- Dermatology
- Cell Biology
Background:
- Keratinocytes are key cells in skin, responding to retinoic acid (RA) signaling.
- Retinoic acid response elements (RAREs) are DNA sequences that regulate gene expression in response to RA.
Purpose of the Study:
- To investigate the binding of specific orphan receptors to RAREs in keratinocytes.
- To determine if ARP subfamily members in keratinocytes interact with RA-regulated response elements.
Main Methods:
- Electrophoretic mobility shift assays (EMSAs) were used to test protein-DNA binding.
- In vitro translated proteins (ARP1, EAR3, EAR2) and keratinocyte nuclear extracts were utilized.
- Specific RAREs (VLRRE2 and RARE beta) were investigated.
Main Results:
- In vitro translated ARP1, EAR3, and EAR2 bound to both VLRRE2 and RARE beta.
- An anti-ARP antibody confirmed that ARP subfamily members in keratinocytes bind to these RAREs.
- This is the first report of these specific protein-RARE interactions in keratinocytes.
Conclusions:
- ARP subfamily members (ARP1, EAR3, EAR2) bind to RA-regulated RAREs in keratinocytes.
- These findings suggest a potential role for ARP subfamily members in modulating retinoic acid-mediated transcription in the epidermis.