Sodium-coupled transport of glucose by plasma membranes of type II pneumocytes

Sodium-dependent absorption of alveolar fluid promotes efficient gas exchange. In animal models, alveolar glucose stimulates phlorizin-sensitive, Na(+)-dependent fluid absorption. It is hypothesized that Na+/glucose cotransporters are localized to apical membranes of type II pneumocytes. Enriched apical and basolateral plasma membrane vesicles were isolated from adult bovine type II pneumocytes. Uptakes of 22Na+ and [3H]glucose by enriched apical and basolateral vesicles were monitored over time. Following addition of external glucose (75 mM), 22Na+ uptake by mannitol-loaded, apically-enriched vesicles was significantly increased over controls. Substitution of interior-negative charge gradients for internally directed Na+ gradients increased glucose-dependent Na+ uptakes even greater. By contrast, external glucose did not significantly promote 22Na+ uptake by enriched basolateral vesicles. External Na+ (75 mM) significantly increased [3H]glucose uptakes by enriched apical vesicles with evidence of overshoot. Phlorizin (100 microM) inhibited both glucose-coupled 22Na+ uptakes and Na(+)-coupled [3H]glucose uptakes. These observations support localization of electrogenic, Na+/glucose cotransporters to enriched apical membranes of mature type II pneumocytes.