Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mapping interaction forces with the atomic force microscope

M Radmacher1, J P Cleveland, M Fritz

  • 1Physics Department, University of California, Santa Barbara 83106.

Biophysical Journal
|June 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shallow coastal zones are key mediators in Arctic land-ocean carbon fluxes.

Communications earth & environment·2025
Same author

[Cognition and driving ability in chronic pain syndrome].

Der Nervenarzt·2022
Same author

Double power-law viscoelastic relaxation of living cells encodes motility trends.

Scientific reports·2020
Same author

Climatic, geomorphologic and hydrologic perturbations as drivers for mid- to late Holocene development of ice-wedge polygons in the western Canadian Arctic.

Permafrost and periglacial processes·2019
Same author

Inorganic Overgrowth of Aragonite on Molluscan Nacre Examined by Atomic Force Microscopy.

The Biological bulletin·2017
Same author

Influence of microenvironment topography and stiffness on the mechanics and motility of normal and cancer renal cells.

Nanoscale·2017
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
Same journal

Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

Biophysical journal·2026
Same journal

Kinesin-5/Cut7 C-terminal tail phosphorylation influence on motor regulation through multi-scale molecular modeling.

Biophysical journal·2026
Same journal

Dynamic conformations of fluorophores on self-labeling protein tags.

Biophysical journal·2026
See all related articles

Atomic force microscopy now allows simultaneous measurement of sample characteristics like topography and elasticity. This technique enhances imaging capabilities for various materials, including thin films and DNA molecules.

Area of Science:

  • Materials Science
  • Surface Science
  • Nanotechnology

Background:

  • Atomic Force Microscopy (AFM) is a high-resolution surface imaging technique.
  • Traditional AFM primarily focuses on topographical imaging.
  • Simultaneous measurement of multiple sample properties can provide deeper insights.

Purpose of the Study:

  • To introduce and demonstrate new imaging opportunities with AFM.
  • To showcase the simultaneous measurement of diverse sample characteristics.
  • To illustrate the application of these new capabilities on various sample types.

Main Methods:

  • Recording force curves during raster-scanning of samples under the AFM tip.
  • Utilizing force-distance spectroscopy to probe surface interactions.

Related Experiment Videos

  • Developing imaging protocols for simultaneous data acquisition.

    • Main Results:

    • Demonstrated simultaneous measurement of topography, adhesion forces, elasticity, van der Waals, and electrostatic interactions.
    • Generated multi-modal images of thin metal films, lysozyme aggregates, and single DNA molecules.
    • Validated the expanded capabilities of AFM for comprehensive material characterization.

    Conclusions:

    • AFM force curve analysis opens new avenues for multi-modal surface characterization.
    • The presented methods enable simultaneous acquisition of diverse physical properties.
    • This advancement significantly enhances the utility of AFM for analyzing complex nanoscale systems.