Analysis of the in vivo myogenic status of chick somites by desmin expression in vitro

Expression of the muscle specific intermediate filament protein, desmin, is an early marker for chick somitic myogenesis. Somites are transient, paired, mesodermal structures adjacent to the neural tube which are formed very uniformly in a cranial to caudal fashion. The developmental somitic expression of desmin in vivo has been reported previously (Holtzer et al. [1991] "Frontiers in Muscle Research." New York: Elsevier Science, pp 187-207; Borman and Yorde [1994] J. Histochem. Cytochem. 42:265-272). Here we explore the ability of those somitic cells which are desmin negative in vivo to successfully carry out a myogenic program of development in the absence of the surrounding embryonic microenvironment. Somites which are known to be overtly desmin negative in the embryo were explanted and cultured on collagen gels for 4 days. Immuno-detection of desmin identified a population of somites that could support desmin positive cells in vitro as well as a population of somites that could not. The cranially located somites must remain in the embryo for a greater length of time than the caudally positioned somites prior to each being able to express desmin in vitro. In embryos of many ages there is also a population of somites unable to support desmin expression in vitro. The rate at which this ability to support somitic desmin expression in vitro progresses caudally in the embryo is significantly greater than the rate at which somites form. Notably, the detected expression of desmin in somites in vitro is parallel to the rate at which overt expression of desmin in vivo is detected. The implication for these observations with regard to the regulation of somitic myogenesis is discussed.