Crystallization and preliminary x-ray investigation of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens

P-Hydroxybenzoate hydroxylase (EC 1.14.13.2) can be crystallized from 0.1 M potassium phosphate buffer, pH 7.5, 39% saturated ammonium sulfate, and 1mM p-hydroxybenzoate. The space group is C2221 with 8 molecules/unit cell. When p-hydroxybenzoate is removed, the diffraction pattern and the cell dimensions change.