Related Experiment Videos
Inhibition of c-Fos protein expression in rat spinal cord by antisense oligodeoxynucleotide superfusion
F Gillardon1, H Beck, E Uhlmann
1II. Physiologisches Institut, Universität Heidelberg, Germany.
The European Journal of Neuroscience
|May 1, 1994
Abstract:
Peripheral noxious stimulation leads to a rapid and transient expression of c-fos, c-jun and other immediate-early genes (IEGs) in the spinal cord. However, the role of IEG encoded transcription factors in plasticity of spinal neurons remains speculative. In the present study we have shown that superfusion of rat spinal cord with antisense oligodeoxynucleotides complementary to c-fos mRNA suppresses heat-induced c-Fos protein expression without affecting other members of the Fos and Jun family, thus providing a technique to determine the function of IEGs in vivo.