Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Steroid hydroxylations by human adrenal cortex microsomes 1, 2

E B Nelson, G T Bryan

    The Journal of Clinical Endocrinology and Metabolism
    |July 1, 1975
    PubMed
    Summary

    Human adrenal microsomes contain cytochrome P-450 enzymes involved in steroid hydroxylation. These enzymes, particularly 21-hydroxylase and 17-hydroxylase, show specific activity patterns and immunological similarities to other species.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Corrigendum to "Preclinical safety evaluation in rats using a highly purified ethyl ester of algal-docosahexaenoic acid" [Food Chem. Toxicol. 48 (2010) 2778-2784].

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2020
    Same author

    Improved Biocontrol Efficacy of Trichoderma harzianum 1295-22 for Foliar Phases of Turf Diseases by Use of Spray Applications.

    Plant disease·2019
    Same author

    Preclinical safety evaluation in rats using a highly purified ethyl ester of algal-docosahexaenoic acid.

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2010
    Same author

    Fungus gnat feeding and mechanical wounding inhibit Pythium aphanidermatum infection of geranium seedlings.

    Phytopathology·2009
    Same author

    Seed-colonizing microbes from municipal biosolids compost suppress Pythium ultimum damping-off on different plant species.

    Phytopathology·2008
    Same author

    Ecological Studies of Transformed Trichoderma harzianum Strain 1295-22 in the Rhizosphere and on the Phylloplane of Creeping Bentgrass.

    Phytopathology·2008

    Area of Science:

    • Biochemistry
    • Endocrinology
    • Molecular Biology

    Background:

    • Human adrenal microsomes are crucial for steroid hormone synthesis.
    • Cytochrome P-450 enzymes play a vital role in these metabolic pathways.
    • Understanding these enzymes is key to comprehending adrenal function and related disorders.

    Purpose of the Study:

    • To characterize the cytochrome P-450 system in human adrenal microsomes.
    • To investigate the kinetics and substrate specificity of steroid hydroxylation.
    • To compare the human adrenal system with those from other species.

    Main Methods:

    • Preparation and assay of human adrenal microsomes.
    • Measurement of cytochrome P-450 concentrations and NADPH-cytochrome c reductase activity.
    • Steroid hydroxylation rate determination and substrate binding studies.
    • Immunological inhibition using antibodies against NADPH-cytochrome c reductase.

    Main Results:

    • Human adrenal microsomes exhibit specific rates for 21- and 17-hydroxylation of progesterone and 17-hydroxyprogesterone.
    • Progesterone and 17-hydroxyprogesterone bind to microsomes exhibiting a type I spectrum.
    • Antibodies against porcine NADPH-cytochrome c reductase inhibited human adrenal reductase and hydroxylation activities.
    • No evidence for multiple 21-hydroxylase forms was found in human adrenal microsomes.

    Conclusions:

    • The human adrenal microsomal cytochrome P-450 electron transport chain is immunologically similar to those in beef adrenal and rat/human liver.
    • These findings contribute to the understanding of steroidogenesis and potential drug interactions involving cytochrome P-450 enzymes.

    Related Experiment Videos